LSPR Biosensors

LamdaGen offers its nano-structured LSPR continuous metallic thin film biosensors both as a consumable for its LightPath™ systems and also as independent biosensors for researchers who wish to work with a highly reproducible and commercially available LSPR biochip.

Many researchers have available their own experimental set-ups and LamdaGen’s LSPR biosensors are easily integrated for immediate use.  These highly sensitive and robust LSPR biosensors offer significant ease of use and performance advantages relative to the often involved processes required with metal colloidals or in the creation and use of other LSPR type active surfaces.

We offer a range of standard sized LSPR biosensors as well as highly customized formats that can work with many different type set-ups and applications.

Researchers are using LamdaGen’s LSPR sensors for work in labeled and non-labeled biomolecular monitoring, drug discovery, diagnostics, SERS, MALDI-TOF, imaging and chemical sensing to name a few.

LamdaGen produces several varieties  of nano based LSPR biochips and sensors for use in Diagnostics and Life Science Research.

For information on purchasing LSPR sensors please email: info@lamdagen.com

 

Pre-functionalized LSPR Biosensors – Surface Functionalization

Our standard sized LSPR sensors are available either with or without surface functionalization. Commonly, the sensor surface is prepared with a self-assembled monolayer (SAM) formed by a mixture of hydrophobic and hydrophilic n-alkanethiols. The hydrophilic alkanethiols are terminated with various functional groups.

We have also evolved a number of different surface protocols for optimization of differing assay models. The most requested surfaces are listed below.

Contact us for more information info@lamdagen.com

Sensor Chemistry Description
Standard Non-functionalized LSPR surface
Carboxyl -COOH groups are activated with EDC/NHS to form covalent binding with NH2 on the ligand
Amine -NH2 group allows forming covalent binding with COOH on the ligand via amide bond activated by EDC/NHS
Thiol -SH on the surface to link to NH2 on the ligand through SMCC-like bioconjugation approach
Protein A Binding with high affinity to Fc region of human IgGs.  Other IgG species may vary.
Streptavidin High binding affinity with biotinylated molecules, including peptides, oligonucleotides, proteins and sugars
Custom Available upon request. Contact: info@lamdagen.com

Frequently Asked Questions

FAQ: How reproducible are LamdaGen’s LSPR biosensors?

At LamdaGen’s nano-fabrication facility, the Company’s manufacturing processes have been diligently altered and refined over the years into very specific protocols and procedures that ensure precise reproducibility of its nano-structured surfaces – sensor to sensor, chip to chip, batch to batch and month to month. Additionally, due the fact that the biosensors are manufactured as metallic thin-films, the stability of LamdaGen biosensors has been evidenced to exceed four years.

FAQ: Can LamdaGen’s LSPR films be grown on differing substrate materials?

LamdaGen’s manufacturing processes and protocols are highly controllable, robust and reproducible.  By precisely altering certain conditions, the Company has learned how to “grow” its LSPR continuous metallic films on almost any surface.  Presently we are manufacturing for end users, including academic researchers, on many polymers, ceramics, as well as stainless and glass for a multiplicity of uses.  Recently we are also growing films on selected membranes and filters for use in the area of diagnostics. Contact us for custom requests.

FAQ: Are LamdaGen’s LSPR biosensors scalable for throughput?

LamdaGen’s LSPR biosensors are a continuous metallic thin film which is characterized by a variety of unique physical, optical, and electrical properties that can be precisely controlled and tuned during manufacture for specific uses. These patented nano-structured films are “grown” on most any surface (glass-polymers-stainless-ceramic etc) and to nearly any size, shape or geometry, again depending on need or use. Due to this flexibility, the Company’s robust biosensors are also quite scalable.

 
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